In Vitro Multiple Shoot Induction From Embryonic Axes of Annual Herbaceous Legume Faba Bean (Vicia faba L.)

The faba bean (Vicia L.) is cultivated in the Mediterranean and Aegean regions only. It a rich source of protein an important food feed for human animal consumption. Faba beans have narrow genetic base their improvement through hybridization not feasible because high pollen self-incompatibility. This study was carried out using embryonic axis cv. Filiz99 Eresen87 regenerated on MS medium containing 11 different combinations BAP NAA. results showed 100% shoot regeneration frequency with maximum number 3.3 3.5 shoots per explant respectively. Regenerating were rooted 1 mg L–1 IAA. vitro continuously cultured 3 weeks to acclimatize them. approach could improve broad seed germination subsequently regeneration. also facilitate transformation studies.